# PREPARATION OF A RECOMBINANT SPIDER SILK PROTEIN/PCL BLEND SUBMICROFIBROUS MAT AND ITS CYTOCOMPATIBILITY

> Title: PREPARATION OF A RECOMBINANT SPIDER SILK PROTEIN/PCL BLEND SUBMICROFIBROUS MAT AND ITS CYTOCOMPATIBILITY Page Range: p.85-92 Author(s): Liang Zhao; Denglong Chen; Meihong Wei; Qinghua Yao; Min Li File size: Download the pdf (subscribers only) Buy the pdf (non-subscribers) Journal: Polymers and...

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Published: 2013-02-01T00:00:00+00:00
Categories: Polymers and Polymer Composites

**Title: PREPARATION OF A RECOMBINANT SPIDER SILK PROTEIN/PCL BLEND SUBMICROFIBROUS MAT AND ITS CYTOCOMPATIBILITY**
 Page Range: p.85-92
 Author(s): Liang Zhao; Denglong Chen; Meihong Wei; Qinghua Yao; Min Li
 File size:
 ![](http://dload.gif) [Download the pdf](http://journals.asp?Page=111&JournalDownload=ON&JournalType=ppc&JournalIssue=ppc21-2&JournalID=101829&JIP=) (subscribers only)
 ![](http://handbuy.gif) Buy the pdf (non-subscribers)
 Journal: Polymers and Polymer Composites
 Issue Year: ppc
 Volume: 21
 Issue No: No.2

**Abstract**
 With electrospinning technology, recombinant spider silk protein/PCL blend submicrofibrous mat was prepared. Its properties as a potential wound dressing material were evaluated by Scanning Electron Microscopy (SEM) and Fourier Transform Infrared Spectroscopy (FTIR). When the proportion of recombinant spider silk protein/ polycaprolactone (PCL) reached 1:20, the appropriate concentration of spinning solution was 30%. Also, the fibre diameter increased as the nozzle-to-ground distance decreased, or as the extrusion speed increased. The fibres tended to be non-uniform. As shown by FTIR, PCL and recombinant spider silk protein blended together without obvious molecular interaction. MTT assay analysis showed that, compared with PCL scaffold, the toxic level of pNSR16/PCL composite scaffold was below 1, which meets the criteria for biomaterials. NIH-3T3 cells cultivation on the composite scaffolds indicated that pNSR16/PCL composite scaffolds with RGD had better cell adhesion than PCL scaffolds. Immunohistochemical experiments proved that cells on the pNSR16/PCL composite scaffold materials had no effect on normal growth. pNSR16/PCL composite scaffold guaranteed good crawling capacity of cells. 22 Refs.

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