# IN-VITRO RESPONSE OF FIBROBLASTS TO POLY-L-LACTIDE COMPOSITE MATERIALS CONTAINING BOVINE BONE

> Title: IN-VITRO RESPONSE OF FIBROBLASTS TO POLY-L-LACTIDE COMPOSITE MATERIALS CONTAINING BOVINE BONE Page Range: p.531-536 Author(s): Zeqiang He; Lizhi Xiong File size: 328K Download the pdf (subscribers only) Buy the pdf (non-subscribers) Journal: Polymers and Polymer Composites Issue Year: ppc Volume: 20...

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Published: 2012-06-01T00:00:00+00:00
Categories: Polymers and Polymer Composites

**Title: IN-VITRO RESPONSE OF FIBROBLASTS TO POLY-L-LACTIDE COMPOSITE MATERIALS CONTAINING BOVINE BONE**
 Page Range: p.531-536
 Author(s): Zeqiang He; Lizhi Xiong
 File size: 328K
 ![](http://dload.gif) [Download the pdf](http://journals.asp?Page=111&JournalDownload=ON&JournalType=ppc&JournalIssue=ppc20-6&JournalID=101653&JIP=) (subscribers only)
 ![](http://handbuy.gif) Buy the pdf (non-subscribers)
 Journal: Polymers and Polymer Composites
 Issue Year: ppc
 Volume: 20
 Issue No: No.6

**Abstract**
 This study aimed to investigate the interaction between fibroblastic cells and poly-L-lactide (PLLA) composite film containing bovine bone. The effects of PLLA/bovine bone on cell cytotoxicity, attachment, proliferation and differentiation of fibroblasts in-vitro were investigated. The results of cytotoxicity tests showed that cells cultured in extracts of PLLA/bovine bone and on the surface of composites showed normal growth and proliferation, and mineralisation nodules were observed for fibroblasts cultured in PLLA/bovine bone extract at day 7 by using von Kossa staining and tetracycline staining. Compared with pure PLLA materials, bovine bone in the PLLA composites facilitated both adhesion and proliferation of rat fibroblasts on the PLLA/bovine bone composite film. In conclusion, these results suggest that the introduction of bovine bone may improve the cell response involved in the osteogenesis and thus may be beneficial to further modify poly-L-lactide as a better bone repairing material. 15 Refs.

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